y 89  (fluidigm)

Journal: iScience

Article Title: PD-L1 + CD8 + T cells enrichment in lung cancer exerted regulatory function and tumor-promoting tolerance

doi: 10.1016/j.isci.2022.103785

Figure Lengend Snippet: Properties of immune infiltrate in human lung cancer determined with CyTOF (A) Schematic diagram of the experimental design and analysis. Adjacent lung tissues and tumor tissues were collected from lung cancer patients undergoing surgical resection of primary tumors, processed barcoded, and stained with antibodies conjugated to metal isotopes. Single-cell mass cytometry data were clustered using Phenograph to identify common populations across patients. (B) viSNE analysis of CD45 + immune cells colored and labeled by tissue types (left) and Phenograph (right). (C) Heatmap of Phenograph clusters of CD45 + cells. The relative expression levels of markers across cells were shown, sorted by cell type. (D) viSNE analysis of primary immune cells colored by the relative expression of CyTOF markers. (E) Cell-type fractions in lung cancer tumors and adjacent tissues, colored by cell types (N = 15). (F) Composition of the CD45 + compartment showing average frequencies of major immune lineages for each tissue across patients, and bar plots showing the frequencies for each patient (N = 15). Bar plots show the mean ± SEM. (G) Normalized expression of PD-L1 on CD45 + cells shown in a viSNE plot. Bar plots show the mean ± SEM ∗p <0.05 and ∗∗p <0.01, paired two-tailed t-test.

Article Snippet: anti-human CD45 -89Y , Fluidigm , Cat# 3089003B.

Techniques: Staining, Mass Cytometry, Labeling, Expressing, Two Tailed Test

Journal: iScience

Article Title: PD-L1 + CD8 + T cells enrichment in lung cancer exerted regulatory function and tumor-promoting tolerance

doi: 10.1016/j.isci.2022.103785

Figure Lengend Snippet: The IMC system analysis indicated PD-L1 + CD8 + T cells enrichment in lung tumor tissues and spatially located with PD-1 + effect T cells (A) Staining for tumor cells (Pan-CK), immune cells (CD45, CD3, CD4, and CD8), and nuclei (DNA) in one of the representative patients. (B) Normalized expression of CD45, Pan-CK, Epcam, CD3, CD4, and CD8 was shown in the viSNE plot. (C) The intensity of Pan-CK + , Epcam + , CD45 + , and CD3 + cells in lung tumors and adjacent tissues, N = 9. (D) Representative co-expression of CD8 and PD-L1 in tumor tissues from two patients. (E) viSNE plot of CD8 + T cells, showing the identification of 11 clusters. Each dot corresponds to a single cell, colored according to the cell cluster. (F) Heatmap of the Phenograph clusters of CD8 + cells. The relative expression levels of markers across cells were shown. (G) The frequency of PD-L1 + , PD-1 + , CD38 + , and CD39 + in CD8 + T cells in adjacent and tumor tissues is determined by IMC. N = 9, two tailed paired t-test. (H) Two representative samples of the neighborhood cells with PD-L1 + CD8 + T cells in lung tumor tissues. (I) viSNE plot showed the neighborhood cells with PD-L1 + CD8 + T cells in lung tumor tissues, including 7 clusters. (J) viSNE plot of the related markers of the CD8 + T cell clusters generated in (I). (K) Re-cluster of neighborhood CD8 + T cells with PD-L1 + CD8 + T cells in lung tumor tissues, including five clusters. (L) Heatmap of the related markers across the neighborhood CD8 + T cells generated in (K).

Article Snippet: anti-human CD45 -89Y , Fluidigm , Cat# 3089003B.

Techniques: Staining, Expressing, Two Tailed Test, Generated

Journal: iScience

Article Title: PD-L1 + CD8 + T cells enrichment in lung cancer exerted regulatory function and tumor-promoting tolerance

doi: 10.1016/j.isci.2022.103785

Figure Lengend Snippet:

Article Snippet: anti-human CD45 -89Y , Fluidigm , Cat# 3089003B.

Techniques: Recombinant, Antibody Labeling, Enzyme-linked Immunosorbent Assay, Sequencing, Software